Elitech UK Ltd - niche products to clinical markets throughout the UK
  • About us
  • Products
  • News
  • Manufacturers
  • Contact us
  • Home
      ELITe MGB™ 2012 Brochure
    • Leading edge assays with proprietary technology
    • Higher sensitivity
    • Monoreagent format
    • Universal cycling conditions
    • Melt-curve analysis
      Innovation in Real-Time PCR
    Product Code Product description Qty
    RTK015PLD CMV ELIte MGB™ Kit 100
    STD015PLD CMV ELITe Standard 8
    RTS020PLD EBV ELITe MGB™ Kit 100
    STD020PLD EBV ELITe Standard 16
    CTR020PLD EBV ELITe Positive Control 12
    RTS031PLD HSV1 ELITe MGB™ Kit 100
    STD031PLD HSV1 ELITe Standard 16
    CTR031PLD HSV1 ELITe Positive Control 12
    RTS032PLD HSV2 ELITe MGB™ Kit 100
    STD032PLD HSV2 ELITe Standard 16
    CTR032PLD HSV2 ELITe Positive Control 12
    RTS035PLD VZV ELITe MGB™ Kit 100
    STD035PLD VZV ELITe Standard 16
    CTR035PLD VZV ELITe Positive Control 12
    RTS036PLD HHV6 ELITe MGB™ Kit 100
    STD036PLD HHV6 ELITe Standard 16
    CTR036PLD HHV6 ELITe Positive Control 12
    RTS038PLD HHV8 ELITe MGB™ Kit 100
    STD038PLD HHV8 ELITe Standard 16
    CTR038PLD HHV8 ELITe Positive Control 12
    RTS070PLD Parvovirus B19 ELITe MGB™ Kit 100
    STD070PLD Parvovirus B19 ELITe Standard 16
    CTR070PLD Parvovirus B19 ELITe Positive Control 12
    STD078PLD ADENOVIRUS ELITe Standard 16
    CTR078PLD ADENOVIRUS ELITe Positive Control 12
    RTS175PLD BKV ELITe MGB™ Kit 100
    STD175PLD BKV ELITe Standard 16
    CTR175PLD BKV ELITe Positive Control 12
    • MTB ELITe MGB™ Kit
      Please visit www.elitechgroup.com/elitechmgblegalnotice for complete licensing and warranty information.
      Leading edge assays with proprietary technology
      ELITe MGB™ is a revolutionary advance in Real-Time PCR chemistry. All ELITe MGB™ assays have been designed with our proprietary Minor Groove Binder (MGB) protein, Superbases™ and Eclipse®Dark Quencher technologies.

    Our patented technology features shorter, overlapping probes that efficiently and accurately detect target DNA sequences, while offering greater sensitivity and specificity.

    The MGB protein is a synthetic molecule that binds to the minor groove of double stranded DNA molecules. In Real-Time PCR applications, MGB increases the stability of double stranded DNA complexes, specifically, the hybridization between the probe and the amplified DNA target. The increased DNA-DNA hybrid stability allows the design of shorter detection probes with higher specificity. Furthermore, the specificity of ELITe MGB™ probes increases the ability to discriminate between a perfectly matched sequence and a mismatched target compared to analogous MGB-free, longer counterpart probes.
      Superbases™ are specially engineered, nitrogen-based nucleotides. The proprietary design of these nucleotides minimizes secondary and tertiary structure observed in A-T or G-C rich DNA target sequences. This feature allows ELITe MGB™ assays to target even the most challenging gene sequences without compromising accuracy, clinical specificity or sensitivity.

    The Eclipse®Dark Quencher is a proprietary fluorophore and dye quencher chemistry resulting in low background signals. Its key benefit is to ensure that every ELITe MGB™ assay will have the highest sensitivity by minimizing background signal interference.
      ELITe MGBprobes feature higher sensitivity, specificity and low fluorescence background.
      Universal Cycling Conditions
      All the ELITe MGBTM assays utilize the same temperature and amplification profile. Known commonly as Universal Cycling Conditions, this profile consists of a three-step protocol: a denaturing step, an annealing step and an extension step. Universal Cycling Conditions enable laboratories to create multiple targets runs that can be run simultaneously on the same thermocycler.

    Denaturation step: The coiled ELITe MGBTM probe float freely in solution and will not emit fluorescence due to the close proximity of the fluorophore and dye-quencher.

    Annealing step: The probe anneals to the target DNA sequence. In this conformation the fluorophore is separated from the quencher and emits light at a specific wavelength.

    Extension step: During DNA polymerization, the probe is displaced from the target gene sequence and reverts to its coiled-conformational state without being hydrolyzed. In this state, the probe is free in solution and will not emit fluorescence due to the close proximity of the fluorophore and dye-quencher.
      Monoreagent format
      ELITe MGB™ mastermix has been reformulated to allow for a larger sample volume, significantly improving assay sensitivity. ELITe MGB™ mastermix is provided as a single solution in a ready-to-use format and includes all the components required for Real-Time PCR. No pipetting, mixing or reagent set-up is necessary; simply aliquot mastermix into the reaction wells and add your extracted sample.
      Melt-curve analysis
      The melt-curve analysis is a technique that monitors the relative fluorescence from the ELITe MGB™ probe as the temperature is gradually increased. This process creates a sequence specific dissociation profile, or melt-curve fingerprint, that is unique for a given doublestranded DNA fragment. For laboratories to utilize this valuable tool, Real-Time PCR methods must use DNA probes that are intact and undegraded. During Real-Time PCR, ELITe MGB™ Probes remain intact (unlike older Real- Time methods in which probes are degraded).

    At low temperature, ELITe MGB™ probe is hybridized to the DNA target generating maximum fluorescence. Increasing the temperature causes the ELITe MGB™ probe to dissociate or “melt” from the DNA target. As the ELITe MGB™ probe “melts” the fluorophore and quencher are brought back in proximity resulting in lower fluorescence. See upper graph at left.

    Creating a second graphic (first derivative) will identify the specific Melt Temperature (TM) corresponding to the inflection point in the first graphic.

    Because melt-curve analysis can resolve subtle pairing differences between the probe and target sequences, it is a valuable tool to identify clinically relevant DNA sequence alterations. A mismatch pairing could result in an underestimation of the quantity of bacterial or viral target present in the clinical sample. By analyzing the melt-curve profile at the end of each amplification, the accuracy, specificity and integrity of the quantitative analysis can be assessed.

    As such, melt-curve analysis gives clinicians critical data that could be clinically or therapeutically important or impact the course of care for their patients.